Journal
ELIFE
Volume 6, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.25690
Keywords
-
Categories
Funding
- American Heart Association [16POST31150011]
- Canadian Institutes of Health Reseach [MOP-81142]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2015-06266]
- Natural Sciences and Engineering Research Council of Canada
- National Institutes of Health [1U01NS090600, K99 HL133473, HL133272, R01 EY022638, R21 NS081507]
- National Science Foundation [1707359]
- Rita Allen Foundation
- Robert and Janice McNair Foundation
- Stanford University
- The Walter V. and Idun Berry
- Universite Laval Neuroscience Thematic Research Centre
- [Burroughs Wellcome Fund]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1707359] Funding Source: National Science Foundation
Ask authors/readers for more resources
Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise for optical detection of voltage transients, many indicators exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short of visualizing voltage dynamics in individual neurons in single trials. Here, we report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila. These results demonstrate that the combination of ASAP2s and fast two-photon imaging methods enables detection of neural electrical activity with subcellular spatial resolution and millisecond-timescale precision.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available