Journal
ELIFE
Volume 6, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.23136
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Funding
- Helmholtz-Gemeinschaft [VH-NG-932]
- Max-Planck-Gesellschaft
- National Institutes of Health [R01GM081747]
- European Commission [334030]
- Japan Society for the Promotion of Science [25000013, 26293097]
- Natural Sciences and Engineering Research Council of Canada
- Alexander von Humboldt-Stiftung
- Ministry of Education, Culture, Sports, Science and Technology [24117004, 15H01640]
- Direct For Mathematical & Physical Scien
- Division Of Mathematical Sciences [1515130] Funding Source: National Science Foundation
- Grants-in-Aid for Scientific Research [15H01640, 26293097, 24117004, 25000013] Funding Source: KAKEN
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The bacterial flagellum is a self-assembling nanomachine. The external flagellar filament, several times longer than a bacterial cell body, is made of a few tens of thousands subunits of a single protein: flagellin. A fundamental problem concerns the molecular mechanism of how the flagellum grows outside the cell, where no discernible energy source is available. Here, we monitored the dynamic assembly of individual flagella using in situ labelling and real-time immunostaining of elongating flagellar filaments. We report that the rate of flagellum growth, initially similar to 1,700 amino acids per second, decreases with length and that the previously proposed chain mechanism does not contribute to the filament elongation dynamics. Inhibition of the proton motive force-dependent export apparatus revealed a major contribution of substrate injection in driving filament elongation. The combination of experimental and mathematical evidence demonstrates that a simple, injection-diffusion mechanism controls bacterial flagella growth outside the cell.
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