Journal
ELIFE
Volume 6, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.27414
Keywords
-
Categories
Funding
- Northwestern University
- Chicago Community Trust
- American Cancer Society [IRG-15-173-21]
- SUNY Research Foundation
- Central New York Community Foundation
- National Cancer Institute [5K22CA184235]
- National Institute of General Medical Sciences [GM110387]
- National Science Foundation [MCB-1149521]
- Alexandrine and Alexander L. Sinsheimer Fund
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1149521] Funding Source: National Science Foundation
Ask authors/readers for more resources
Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 A resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from -27 to -16. Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available