Journal
APPLIED SOIL ECOLOGY
Volume 111, Issue -, Pages 114-122Publisher
ELSEVIER
DOI: 10.1016/j.apsoil.2016.12.003
Keywords
Black soil; Fungal community structure; Long-term fertilization; Manure amendment
Categories
Funding
- National Natural Science Foundation of China [41573066]
- National Key Basic Research Program of China (973 Program) [2015CB150506]
- Special Fund for China Agriculture Research System [CARS-04]
- National High Technology Research and Development Program of China [2013AA102802-04]
Ask authors/readers for more resources
The effects of 35 years of manure amendment on fungal communities were evaluated in Mollisols of northeast China. Soil samples from different fertilization regimes were collected, and quantitative PCR analysis of fungal community size and Illumina platform based analysis of the ITS gene were performed to characterize soil fungal abundance and compare community structure and diversity. The treatments were no fertilizer (CK); manure (M); nitrogen, phosphorus and potassium inorganic fertilizer (NPK); and inorganic fertilizer plus manure (MNPK) regimes. Soil fungal diversity was decreased by inorganic fertilizer. Inorganic fertilizer plus manure induced a weak increase in fungal diversity and a slight decrease in size. The predominant phyla were Ascomycota (63.77-78.70%), Zygomycota (8.33-14.80%) and Basidiomycota (4.03-13.47%). At each taxonomic level, the percentages dramatically differed, especially between MNPK and NPK. For example, the numbers of Fusarium and Gibberella with potential pathogenicity were all higher in NPK than in MNPK; the beneficial genus Podospora was the highest in MNPK and the least in NPK. Principal coordinates analysis showed that CK and M were clustered together; the incorporation of NPK with manure improved the fungal structure near to that of CK and separate from that of NPK. Redundancy analysis indicated that fungal community structure was most affected by soil available phosphorus (AP) and organic matter contents, followed by soil pH. Simpson and Shannon indices were closely correlated with soil pH, AP, total phosphorus and total nitrogen. The results indicated that manure application altered soil properties and soil fungal community structure, and manure with inorganic fertilizer counteracted some of the adverse effects of the inorganic fertilizer. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available