Journal
BRAIN RESEARCH
Volume 1658, Issue -, Pages 60-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2017.01.018
Keywords
Astrocytes; TLR3; IFN; IFI35; RIG-I; Chemokine
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Funding
- Setsurou Fujii Memorial Osaka Kisoigaku Kenkyu Shoreikai, Hirosaki University Institutional Research Grant
- Grant for the Special Project of Three Northern Tohoku Universities
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Interferon (IFN)-stimulated genes (ISGs) exert multiple functions in immune system. IFN-induced protein 35 (IFI35) is a member of ISGs, and has been suggested to regulate innate immune reaction. However, the physiological functions and pathological roles of IFI35 in the central nervous system are not characterized well. In the present study, we found that the expression of IFI35 was induced by a Toll-like receptor 3 (TLR3) ligand polyinosinic-polycytidylic acid (poly IC) in U373MG human astrocytoma cells. Knockdown of IFI35 using RNA interference resulted in increased expression of IFN-beta, phosphorylated STAT1 (P-STAT1), retinoic acid-inducible gene-I (RIG-I), CXCL10 and CCL5 induced by poly IC. Poly IC-induced expression of OCCL10 and CCL5 was decreased by knockdown of RIG-I. These results suggest that IFI35 may negatively regulate the TLR3-IFN-beta-P-STAT1-RIG-I-CXCL10/CCL5 axis in U373MG cells, and IFI35 may play a role at least partially in the regulation of innate immune reactions in astrocytes. (C) 2017 Elsevier B.V. All rights reserved.
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