Journal
NATURE PLANTS
Volume 3, Issue 3, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nplants.2017.18
Keywords
-
Categories
Funding
- National Science Foundation of China [31330017, 31271420, 31371682]
- National Transgenic Major Project [2014ZX0801003B-002]
- North Carolina Biotechnology Center
- Syngenta Biotechnology [2016-CFG-8003]
- University of Maryland-College Park
- National Science Foundation [MCB 0209818, DBI 0923827, 105-1339209]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1339209] Funding Source: National Science Foundation
Ask authors/readers for more resources
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice To transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR-Cpf1 for editing plant genomes and modulating the plant transcriptome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available