Journal
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY
Volume 137, Issue -, Pages 318-326Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.dsr2.2016.03.011
Keywords
Bivalve; Bathymodioline; Bathymodiolus; Population genetics; Restriction site-associated DNA; RAD-seq
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Funding
- Hong Kong Baptist University [FRG2/14-15/002]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB06010102]
- State Key Program of National Natural Science of China [31130054]
- Trans-crustal Advection and In situ Biogeochemical Processes of the Global Sub-seafloor Aquifer (TAIGA) Project
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) [20109004]
- Canon Foundation
- Inter Ridge Fellowship
- Grants-in-Aid for Scientific Research [26291077] Funding Source: KAKEN
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The present study aimed to generate genome-wide single nucleotide polymorphisms (SNPs) for the deep-sea mussel Bathymodiolus platifrons via a combination of genome survey sequencing and the type IIB endonuclease restriction-site associated DNA (2b-RAD) sequencing, assess the potential use of SNPs in detecting fine-sale population genetic structure and signatures of divergent selection, as well as their cross-species application in other bathymodioline mussels. Genome survey sequencing was conducted for one individual of B. platifrons. De novo assembly resulted in 781,720 sequences with a scaffold N50 of 2.9 kb. Using these sequences as a reference, 9307 genome-wide SNPs were identified by 2b-RAD for 28 B. platifrons individuals collected from a seep and a vent population. Among these SNPs, nine outliers showed significant evidence for divergent selection, and their positions in the genes or scaffolds were identified. The FsT estimated based on the putative neutral SNPs was low (0.0126) indicating the two B. platifrons populations having a high genetic connectivity. However, the permutation test detected significant differences (P < 0.00001), indicating the two populations having clearly detectable genetic differentiation. The Bayesian clustering analyses and principle component analyses (PCA) performed based on either the putative neutral or outlier SNPs also showed that these two populations were genetically differentiated. In addition, 2b-RAD was also conducted to detect 10,199, 6429, and 3811 single nucleotide variants (SNVs) respectively in the bathymodioline mussels Bathymodiolus japonicus, Bathymodiolus aduloides and Idas sp. with different phylogenetic distances from B. platifrons. Overall, our study has demonstrated the feasibility and effectiveness of combining genome survey sequencing and 2b-RAD to rapidly generate genomic resources for use in fine-scale population genetic studies, and various cross species applications. (C) 2016 Elsevier Ltd. All rights reserved.
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