Journal
BIO-PROTOCOL
Volume 7, Issue 5, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2167
Keywords
Leaf inoculation; Stomatal defense; Pseudomonas syringae; Foliar internalization; Apoplastic bacterial population
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Funding
- US National Institute of Allergy and Infectious Disease [5R01AI068718]
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Bacterial pathogens must enter the plant tissue in order to cause a successful infection. Foliar bacterial pathogens that are not able to directly penetrate the plant epidermis rely on wounds or natural openings to internalize leaves. This protocol describes a procedure to estimate the population size of Pseudomonas syringae in the leaf apoplast after surface inoculation of Arabidopsis rosettes.
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