Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 5, Pages 2972-2977Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b04432
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Funding
- Grant Agency of the Czech Republic (GACR) [15-09368Y, P205-12-G118]
- Ministry of Education, Youth and Sports of CR [BIOCEV-FAR LQ1604]
- project 'BIOCEV' from the European Regional Development Fund [CZ.1.05/1.1.00/02.0109]
- OPPK from the European Regional Development Fund [CZ.2.16/3.1.00/21545]
- Austrian Science Fund (FWF) through the project ACTIPLAS [P 244920-N20]
- FEMTECH Frauen in Forschung and Technologie Initiative of the Austrian Ministry for Transportation, Innovation and Technology [840988]
- Center for Chemical Polymer Technology (CPT) - EU
- federal state of North Rhine-Westphalia [EFRE 30 00 883 02]
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A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.
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