Journal
BIOMATERIALS
Volume 118, Issue -, Pages 74-83Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.11.036
Keywords
Protein adsorption; Mass spectrometry; Polymer beads; Methacrylic acid; Complement activation
Funding
- Ontario Research Foundation [orf re 3-033]
- Natural Sciences and Engineering Research Council (NSERC)
- NSERC [RGPIN 404842]
- Canadian Foundation for Innovation
- Canadian Institute for Health Research (CIHR)
- CIHR Training Program in Regenerative Medicine
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Polymer beads made of 45% methacrylic acid co methyl methacrylate (MAA beads) promote vascular regenerative responses in contrast to control materials without methacrylic acid (here polymethyl methacrylate beads, PMMA). In vitro and in vivo studies suggest that MAA copolymers induce differences in macrophage phenotype and polarization and inflammatory responses, presumably due to protein adsorption differences between the beads. To explore differences in protein adsorption in an unbiased manner, we used high resolution shotgun mass spectrometry to identify and compare proteins that adsorb from human plasma or serum onto MAA and PMMA beads. From plasma, MAA beads adsorbed many complement proteins, such as Clq, C4-related proteins and the complement inhibitor factor H, while PMMA adsorbed proteins, such as albumin, C3 and apolipoproteins. Because of the differences in complement protein adsorption, follow-up studies focused on using ELISA to assess complement activation. When incubated in serum, MAA beads generated significantly lower levels of soluble C5b9 and C3a/C3a(desarg) in comparison to PMMA beads, indicating a decrease in complement activation with MAA beads. The differences in adsorbed protein on the two materials likely alter subsequent cell-material interactions that ultimately result in different host responses and local vascularization. (C) 2016 Elsevier Ltd. All rights reserved.
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