Journal
CLINICA CHIMICA ACTA
Volume 464, Issue -, Pages 98-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2016.11.018
Keywords
Lipoprotein metabolism; Metabolic syndrome; Hypertriglyceridemia; HDL subclasses; Coronary heart disease; Hypoalphalipoproteinemia; Postprandial state
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Funding
- CONACYT
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Background: Postprandial triglyceridemia may transitory affect the structure of HDL subclasses and probably their antiatherogenic properties but little is known in this field. We analyzed the HDL subclasses lipid content along postprandial period. Methods: Fifteen metabolic syndrome (MS) patients and 15 healthy controls were enrolled. HDL were isolated from plasma samples obtained at fasting and every 2-h up to 8-h, after a 75-g fat meal. Cholesterol (C), triglycerides (TAG), and phospholipid (Ph) plasma concentrations of five HDL subclasses were determined by densitometry of electrophoresis gels enzymatically stained. Results: The increase of postprandial triglyceridemia expressed as the incremental area under the curve (iAUC) was twice in MS patients than in controls. Only large HDL2b-TAG were higher in MS than controls at 4, 6 and 8 h after meal intake, whereas cholesterol of HDL2a, 3a and 3b were lower at 8 h. HDL size distribution shifted towards large HDL and HDL3a-, 3b- and 3c-subclasses had a lower content of cholesterol (estimated by the C-to-Ph ratio) in subjects whose iAUC > 289.5 mg h/dl (n = 15) in comparison with those subjects with iAUC below this cutoff point (n = 15), independently of the MS status and fasting TAG. Triglycerides content of HDL subclasses changed only discreetly along the postprandial period, whereas paraoxonase-1 remained unchanged. Conclusions: A high postprandial triglyceridemia conditions the shift of HDL size distribution towards large particles and the decrease of cholesterol in HDL3 subclasses. These data demonstrate that postprandial hypertriglyceridemia contributes to a transitory hypoalphalipoproteinemia that may increase the risk of cardiovascular disease. (C) 2016 Elsevier B.V. All rights reserved.
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