Journal
ANTIVIRAL RESEARCH
Volume 137, Issue -, Pages 125-130Publisher
ELSEVIER
DOI: 10.1016/j.antiviral.2016.11.016
Keywords
Middle East Respiratory Syndrome coronavirus; Equine immune serum; Immunoglobulin; F(ab ')(2) fragment; Animal model
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Funding
- National Key RD program [2016YFC1200902]
- Ministry of Science and Technology of China [2014ZX09102044-007]
- Municipal Healthcare Joint-Innovation Major Project of Guangzhou [201604020011]
- State Key Laboratory of Respiratory Disease
- National Institutes of Health
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Middle East Respiratory Syndrome (MERS) is a highly lethal pulmonary infection caused by a coronavirus (Coy), MERS-CoV. With the continuing spread of MERS-CoV, prophylactic and therapeutic treatments are urgently needed. In this study, we prepared purified equine F(ab')(2) from horses immunized with MERS-CoV virus-like particles (VLPs) expressing MERS-CoV S, M and E proteins. Both IgG and F(ab')(2) efficiently neutralized MERS-CoV replication in tissue culture. Passive transfer of equine immune antibodies significantly reduced virus titers and accelerated virus clearance from the lungs of MERS-CoV infected mice. Our data show that horses immunized with MERS-CoV VLPs can serve as a primary source of protective F(ab')(2) for potential use in the prophylactic or therapeutic treatment of exposed or infected patients. (C) 2016 Published by Elsevier B.V.
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