Journal
BIOMATERIALS
Volume 119, Issue -, Pages 43-52Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.12.008
Keywords
Adipose tissue-derived stem cells (ASC); Hypothermia; Cold storage; Mitochondrial damage; Chromanol
Funding
- Innovative Action Program Groningen (IAG3)
- 'Samenwerkingsverband Noord Nederland (SNN)' EFRO Tender Valorization
- BioBRUG [BB139]
- Groningen University Institute for Drug Exploration (GUIDE)
- Netherlands Organization for Health Research and Development [916.11.022, 917.16.466, 40-00506-98-9021]
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Encouraging advances in cell therapy research with adipose derived stem cells (ASC) require an effective short-term preservation method that provides time for quality control and transport of cells from their manufacturing facility to their clinical destination. Hypothermic storage of cells in their specific growth media offers an alternative and simple preservation method to liquid nitrogen cryopreservation or commercial preservation fluids for short-term storage and transport. However, accumulation of cell damage during hypothermia may result in cell injury and death upon rewarming through the production of excess reactive oxygen species (ROS). Here, the ability of the cell culture medium additive SUL-109, a modified 6-chromanol, to protect ASC from hypothermia and rewarming damage is examined. SUL-109 conveys protective effects against cold-induced damage in ASC as is observed by preservation of cell viability, adhesion properties and growth potential. SUL-109 does not reduce the multilineage differentiation capacity of ASC. SUL-109 conveys its protection against hypothermic damage by the preservation of the mitochondrial membrane potential through the activation of mitochondrial membrane complexes I and IV, and increases maximal oxygen consumption in FCCP uncoupled mitochondria. Consequently, SUL-109 alleviates mitochondrial ROS production and preserves ATP production. In summary, here we describe the generation of a single molecule cell preservation agent that protects ASC from hypothermic damage associated with short-term cell preservation that does not affect the differentiation capacity of ASC. (C) 2016 Elsevier Ltd. All rights reserved.
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