Journal
BIOTECHNOLOGY JOURNAL
Volume 12, Issue 12, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700062
Keywords
bone reconstruction; hydroxyapatite particles; electrospun nanofibrous scaffold; substance P; stem cell mobilization
Funding
- Korea Health Technology R&D Project - Ministry of Health and Welfare, Republic of Korea [HI13C1479]
- Translational Research Center for Protein Function Control (TRCP) - Ministry of Science, ICT and Future Planning, Republic of Korea [2016R1A5A1004694]
Ask authors/readers for more resources
Classical bone tissue engineering involves the use of culture-expanded cells and scaffolds to produce tissue constructs for transplantation. Despite promising results, clinical adoption of these constructs has been limited due to various drawbacks, including extensive cell expansion steps, low cell survival rate upon transplantation, and the possibility of immuno-rejection. To bypass the ex vivo cell culture and transplantation process, the regenerative capacity of the host is exploited by mobilizing endogenous stem cells to the site of injury. Systemic injection of substance P (SP) induce mobilization of CD29(+) CD105(+) CD45(-) cells from bone marrow and enhance bone tissue regeneration in a critical-sized calvarial bone defect model. To provide an appropriate environment for endogenous stem cells to survive and differentiate into osteogenic lineage cells, electrospun nanofibrous polycaprolactone (PCL) scaffolds are functionalized with hydroxyapatite (HA) particles via a polydopamine (PDA) coating to create highly osteoinductive PCL-PDA-HA scaffolds that are implanted in defects. The combination of the PCL-PDA-HA scaffold and SP treatment enhance in situ bone tissue formation in defects. Thus, this in situ bone regeneration strategy, which combines recruitment of endogenous stem cells from the bone marrow to defective sites and implantation of a highly biocompatible and osteoinductive cell-free scaffold system, has potential as an effective therapeutic in regenerative medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available