Journal
BIOTECHNOLOGY JOURNAL
Volume 12, Issue 10, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700310
Keywords
2-phenylethanol; aromatics; L-phenylalanine; styrene; styrene oxide
Funding
- National Science Foundation [CBET-1511637]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1511637] Funding Source: National Science Foundation
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2-Phenylethanol (2 PE) is a key molecule used in the fragrance and food industries, as well as a potential biofuel. In contrast to its extraction from plant biomass and/or more common chemical synthesis, microbial 2 PE production has been demonstrated via both native and heterologous expression of the yeast Ehrlich pathway. Here, a novel alternative to this established pathway is systematically engineered in Escherichia coli and evaluated as a more robust and efficient route. This novel pathway is constructed via the modular extension of a previously engineered styrene biosynthesis pathway, proceeding from endogenous L-phenylalanine in five steps and involving four heterologous enzymes. This styrene-derived pathway boasts nearly a 10-fold greater thermodynamic driving force than the Ehrlich pathway, and enables reduced accumulation of acetate byproduct. When directly compared using a host strain engineered for L-phenylalanine over-production, preservation of phosphoenolpyruvate, and reduced formation of byproduct 2-phenylacetic acid, final 2 PE titers via the styrene-derived and Ehrlich pathways reached 1817 and 1164 mg L-1, respectively, at yields of 60.6 and 38.8 mg g(-1). Following optimization of induction timing and initial glucose loading, 2 PE titers by the styrene-derived pathway approached 2 g L-1 - nearly a two-fold twofold increase over prior reports for 2 PE production by E. coli employing the Ehrlich pathway.
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