Journal
BIOTECHNOLOGY JOURNAL
Volume 12, Issue 12, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201700073
Keywords
cartilage regeneration; chondrogenic differentiation; co-cultures; electrospinning; stem cells
Funding
- Portuguese Foundation for Science and Technology [SFRH/BPD/73322/2010]
- POPH - QREN - Tipologia 4.1 - Advanced Formation
- Fundo Social Europeu
- MEC national funds
- project SPARTAN [PTDC/CTM-BIO/4388/2014]
- FCT/MEC
- PIDDAC funds
- POLARIS [FP7-REGPOT-2012-2013-1]
- Project New methodologies for the isolation and control of stem cells differentiation using advanced culturing conditions and/or nanomaterials [RL2 - SCN - NORTE-01-0124-FEDER-000018]
- North Portugal Regional Operational Programme (ON.2 - O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF)
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/73322/2010] Funding Source: FCT
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Many efforts are being directed worldwide to the treatment of OA-focal lesions. The majority of those efforts comprise either the refinement of surgical techniques or combinations of biomaterials with various autologous cells. Herein, we tested electrospun polycaprolactone (PCL) nanofibrous meshes for cartilage tissue engineering. For that, articular chondrocytes (hACs) isolated from human osteoarthritic joints and Wharton's Jelly Stem Cells (hWJSCs) are cultured on electrospun nanofiber meshes, without adding external growth factors. We observed higher glycosaminoglycans production and higher over-expression of cartilage-related genes from hWJSCs cultured with basal medium, when compared to hACs isolated from osteoarthritic joints. Moreover, the presence of sulfated proteoglycans and collagen type II is observed on both types of cell cultures. We believe that this effect is due to either the electrospun nanofibers topography or the intrinsic chondrogenic differentiation potential of hWJSCs. Therefore, we propose the electrospun nanofibrous scaffolds in combination with hWJSCs as a viable alternative to the commercial membranes used in autologous chondrogenic regeneration approaches.
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