Journal
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Volume 26, Issue -, Pages 20-26Publisher
UNIV CATOLICA DE VALPARAISO
DOI: 10.1016/j.ejbt.2016.12.005
Keywords
beta-Glucosidase-producing microorganisms; Biotransformation; Ginseng adventitious roots; Ginseng growth cycle; Ginseng secondary metabolites; Ginsenoside CK; Ginsenosides conversion; Rare ginsenosides; Wild ginseng; Ginseng root culture; Chinese medicine
Categories
Funding
- National Natural Science Foundation of China [21662038]
- Natural Science Foundation of Jilin Province of China [20150101184JC]
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Background: Ginsenoside is the most important secondary metabolite in ginseng. Natural sources of wild ginseng have been overexploited. Although root culture can reduce the length of the growth cycle of ginseng, the number of species of ginsenosides is reduced and their contents are lower in the adventitious roots of ginseng than in the roots of ginseng cultivated in the field. Results: In this study, 147 strains of beta-glucosidase-producing microorganisms were isolated from soil. Of these, strain K35 showed excellent activity for converting major ginsenosides into rare ginsenosides, and a NCBI BLAST of its 16S rDNA gene sequence showed that it was most closely related to Penicillium sp. (HQ608083.1). Strain K35 was used to ferment the adventitious root extract, and the fermentation products were analyzed by high-performance liquid chromatography. The results showed that the content of the rare ginsenoside CK was 0.253 mg mL(-1) under the optimal converting conditions of 9 d of fermentation at pH 7.0 in LL medium, which was significantly higher than that in the adventitious roots of ginseng. Conclusion: These findings may not only solve the problem of low productivity of metabolite in ginseng root culture but may also result in the development of a new valuable method of manufacturing ginsenoside CK.
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