Journal
BIOMED RESEARCH INTERNATIONAL
Volume 2017, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2017/2350482
Keywords
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Funding
- Fundamental Research Funds for the Central Universities [2662014BQ024]
- National Natural Science Foundation of China [31272631, 31472254, 31502130]
- Undergraduate Special Science and Technology Innovation of Huazhong Agricultural University [2015BC009]
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The aim of the present study was to determine the anti-inflammatory effect of IFN-tau on endometritis using a mouse model of S. aureus-induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN-tau on S. aureus growth was monitored by turbidimeter at 600 nm. IFN-tau did not affect S. aureus growth. The histopathological changes indicated that IFN-tau had a protective effect on uterus tissues with S. aureus infection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-alpha IL-1 beta and IL-6 was decreased with IFN-tau treatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-tau Furthermore, the western blotting results showed the phosphorylation of I kappa B, NF-kappa B p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN-tau treatment. The results suggested that IFN-tau may be a potential drug for the treatment of uterine infection due to S. aureus or other infectious inflammatory diseases.
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