Journal
BLOOD
Volume 129, Issue 11, Pages 1448-1457Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2016-07-728691
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Funding
- intramural research program of the NIH [NHLBIK99-HL11304]
- NHLBI
- California Institute for Regenerative Medicine [TG2-01159]
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Autologous transplantation of hematopoietic stem and progenitor cells lentivirally labeled with unique oligonucleotide barcodes flanked by sequencing primer targets enables quantitative assessment of the self-renewal and differentiation patterns of these cells in a myeloablative rhesus macaque model. Compared with other approaches to clonal tracking, this approach is highly quantitative and reproducible. We documented stable multipotent long-term hematopoietic clonal output of monocytes, granulocytes, B cells, and T cells from a polyclonal pool of hematopoietic stem and progenitor cells in 4 macaques observed for up to 49 months posttransplantation. A broad range of clonal behaviors characterized by contribution level and biases toward certain cell types were extremely stable over time. Correlations between granulocyte and monocyte clonalities were greatest, followed by correlations between these cell types and B cells. We also detected quantitative expansion of T cell-biased clones consistent with an adaptive immuneresponse. In contrast to recent data from a nonquantitative murine model, there waslittle evidence for clonal succession after initial hematopoietic reconstitution. These findings have important implications for human hematopoiesis, given the similarities between macaque and human physiologies.
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