Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 6, Pages 3716-3723Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00031
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21475049, 31471257, 21275060]
- National Key RAMP
- D Program of China [2016YFF0100801]
Ask authors/readers for more resources
Digital loop-mediated isothermal amplification (dLAMP) is an attractive approach for absolute quantification of nucleic acids with high sensitivity and selectivity. Theoretical and numerical analysis of dLAMP provides necessary guidance for the design and analysis of dLAMP devices. In this work, a mathematical model was proposed on the basis of the Monte Carlo method and the theories of Poisson statistics and chemometrics. To examine the established model, we fabricated a spiral chip with 1200 uniform and discrete reaction chambers (9.6 nL) for absolute quantification of pathogenic DNA samples by dLAMP. Under the optimized conditions, dLAMP analysis on the spiral chip realized quantification of nucleic acids spanning over 4 orders of magnitude in concentration with sensitivity as low as 8.7 x 10(-2) copies/mu L in 40 min. The experimental results were consistent with the proposed mathematical model, which could provide useful guideline for future development of dLAMP devices.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available