Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 118, Issue -, Pages 11-19Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2016.11.007
Keywords
Yeast; Fermentation; Bioconversion; Lipids; Lignocellulosic biomass; Inhibitors; Acetic acid
Funding
- FAPESP (Foundation for Research Support of the State of Sao Paulo)
- CAPES (Coordination for the Improvement of Higher Education Personnel)
- CNPq (National Council for Scientific and Technological Development)
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Sugarcane bagasse (SCB) represents a potentially less expensive and renewable source of fermentable sugars that can be converted into biofuels and chemicals. Microbial conversion of the hemicellulose fraction is essential for increased biofuel production, and microorganisms able to overcome the difficulties imposed by inhibitors generated during hydrolysis of the hemicellulosic fraction are desirable for this purpose. Lipomyces starkeyi, cultivated in hemicellulosic hydrolysate (HH) from sugarcane bagasse composed of 14.0 g/L xylose, 2.9 g/L glucose, 2.6 g/L arabinose, 2.6 g/L acetic acid, 0.04 g/L furfural and 0.02 g/L 5-hydroxymethylfurfural (HMF), was not inhibited by HMF, furfural or acetic acid. Furthermore, the inhibitors were depleted during fermentation, possibly due to yeast metabolism. This assumption was supported by analysis of the L. starkeyi genome, which confirmed the presence of key genes related to inhibitor metabolism. Furthermore, a preliminary fermentation using only acetic acid as the carbon source indicated that L. starkeyi was able to use acetic acid (similar to 3.0 g/L) for cell growth (0.9 g/L) and biosynthesis of lipids (28.4%,w/w). L starkeyi showed favorable and robust characteristics as a promising microorganism able to convert HH sugars into lipids without previous detoxification. (C) 2016 Elsevier B.V. All rights reserved.
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