Journal
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
Volume 96, Issue -, Pages 22-31Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejpb.2015.07.002
Keywords
Virus-like particle (VLP); Bioconjugation; Molecular display; Cell entry peptide; Targeting; Drug delivery
Categories
Funding
- Academy of Finland [136288, 273192, 261285, 272288]
- National Doctoral Programme in Informational and Structural Biology (ISB)
- German Research Foundation [Ta157/9 - SPP 1623]
- Cluster of Excellence Macromolecular Complexes
- Academy of Finland (AKA) [272288, 261285, 261285, 273192, 272288] Funding Source: Academy of Finland (AKA)
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In addition to vaccines, noninfectious virus-like particles (VLPs) that mimic the viral capsid show an attractive possibility of presenting immunogenic epitopes or targeting molecules on their surface. Here, functionalization of norovirus-derived VLPs by simple non-covalent conjugation of various molecules is shown. By using the affinity between a surface-exposed polyhistidine-tag and multivalent tris-nitrilotriacetic acid (trisNTA), fluorescent dye molecules and streptavidin-biotin conjugated to trisNTA are displayed on the VLPs to demonstrate the use of these VLPs as easily modifiable nanocarriers as well as a versatile vaccine platform. The VLPs are able to enter and deliver surface-displayed fluorescent dye into HEK293T cells via a surface-attached cell internalization peptide (VSV-G). The ease of manufacturing, the robust structure of these VLPs, and the straightforward conjugation provide a technology, which can be adapted to various applications in biomedicine. (C) 2015 Elsevier B.V. All rights reserved.
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