Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 19, Pages 2862-2865Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc10032e
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Funding
- ERC starting grant [ERC-2011-StG 280255]
- project NextDx
- European Commission FP7 program
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Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.
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