Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 5, Issue 11, Pages 10840-10848Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.7b02774
Keywords
Photoelectrochemistry; MicroRNA-21; Enzyme-assisted cycle amplification; Exciton-plasmon interactions; Heterojunction
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Funding
- National Natural Science Foundation of China [21675136, 21375114, 21405129]
- Plan for Scientific Innovation Talent of Henan Province [2017JR0016]
- Funding Scheme for the Young Backbone Teachers of Higher Education Institutions in Henan Province [2016GGJS-097]
- Nanhu Young Scholar Supporting Program of XYNU
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Novel exciton-plasmon interactions (EPI) between CdS@g-C3N4 heterojunction and Au@Ag nanoparticles (NPs) was introduced for the first time into the photoelectrochemical (PEC) biosensing system for highly sensitive microRNA-21 detection using duplex-specific nuclease assisted cycle amplification for sensitivity enhancement. The photo electrode of CdS@g-C3N4, nanowires could generate a great photocurrent because of the formation of the p-n heterojunction. Due to the natural absorption overlap, the exciton of CdS@g-C3N4 and the plasmon of Au@Ag NPs could be induced simultaneously to form EPI. Specifically, the perfect overlap of the wide absorption spectrum of Au@Ag NPs with the photoluminescence spectrum of CdS@g-C3N4 allows the resonance energy transfer and EPI between CdS@g-C3N4 nanowire and Au@Ag NPs simultaneously. The effective EPI renders the signal change modulated by the interparticle distance significantly. Such a signaling mechanism was then used to biosensor for microRNA-21 detection, within which the duplex-specific nuclease (DSN) was further introduced to enhance the sensitivity. The constructed PEC biosensor exhibits the sub-fM level (0.05 fM) detection of microRNA-21 with a wide range from 0.1 fM to 1.0 nM. In complex biological samples, the proposed method also possesses good specificity, reproducibility, and stability.
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