Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 12, Pages 10544-10553Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b01513
Keywords
poly(2-hydroxyethyl methacrylate); reductively degradable; oriented porosity; 2-methacryloyloxyethyl phosphorylcholine; 2-(acethylthio)ethyl methacrylate; hydrogel
Funding
- Czech Science Foundation [14-14961S]
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Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (M-w = 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2'-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 mu m and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in alpha-minimum essential medium, respectively.
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