Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 5, Issue 11, Pages 9701-9708Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.7b01294
Keywords
Enzymatic hydrolysis; Lignocellulose; Cellulases; Recycling; Biofuel; Mass balance
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Funding
- Consejo Nacional de Ciencia y Tecnologia of Mexico
- Agriculture and Agrifood Canada
- Natural Science and Engineering Research Council of Canada [RGPIN 138356-08]
- University of British Columbia Chemical and Biological Engineering department
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Enzyme recycling by adsorption from supernatant to fresh substrate is a promising strategy to reduce enzyme expenses and the production cost of lignocellulosic ethanol. The study was performed using oxygen-delignified wheat straw, and the effect of lignin content, enzyme loading, and hydrolysis time on recycling was determined. The percent of recycled cellulases, 0-35% of initial cellulase loading, increased with increasing enzyme loading and hydrolysis time but decreased with increasing lignin content. Cellulose conversions of 10-71% were achieved during the second hydrolysis round using only recycled cellulases indicating the existence of a highly active subset of enzymes. To achieve constant production of sugars during enzyme recycling, fresh cellulases were loaded before the second hydrolysis round to match the cellulase loading used in the first round. Subsequently, similar glucose, xylose, and protein concentrations were obtained at the end of the first and second rounds for all conditions. Recycling mass balances were developed to support future techno-economic analyses to determine the impact of enzyme recycling on the cost of ethanol.
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