Journal
TOXINS
Volume 9, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/toxins9040112
Keywords
Pichia membranifaciens; PMKT; PMKT2; killer toxin; biochemical characteristics; mechanism of action; biocontrol; applications
Categories
Funding
- European Community [AIR CT93-0830]
- Universidad Complutense-Community of Madrid [PR1/03-11632, CCG07-UCM/AGR-2612, GR45/06, GR69/06, GR74/07, GR58/08, GR35/10-A]
- CDTI-MINECO-Centre for the Development of Industrial Technology [IDI-20130192, IDI-20140448, IDI-20160102]
Ask authors/readers for more resources
The killer phenomenon is defined as the ability of some yeast to secrete toxins that are lethal to other sensitive yeasts and filamentous fungi. Since the discovery of strains of Saccharomyces cerevisiae capable of secreting killer toxins, much information has been gained regarding killer toxins and this fact has substantially contributed knowledge on fundamental aspects of cell biology and yeast genetics. The killer phenomenon has been studied in Pichia membranifaciens for several years, during which two toxins have been described. PMKT and PMKT2 are proteins of low molecular mass that bind to primary receptors located in the cell wall structure of sensitive yeast cells, linear (1 -> 6)-beta-D-glucans and mannoproteins for PMKT and PMKT2, respectively. Cwp2p also acts as a secondary receptor for PMKT. Killing of sensitive cells by PMKT is characterized by ionic movements across plasma membrane and an acidification of the intracellular pH triggering an activation of the High Osmolarity Glycerol ( HOG) pathway. On the contrary, our investigations showed a mechanism of killing in which cells are arrested at an early S-phase by high concentrations of PMKT2. However, we concluded that induced mortality at low PMKT2 doses and also PMKT is indeed of an apoptotic nature. Killer yeasts and their toxins have found potential applications in several fields: in food and beverage production, as biocontrol agents, in yeast bio-typing, and as novel antimycotic agents. Accordingly, several applications have been found for P. membranifaciens killer toxins, ranging from pre- and post-harvest biocontrol of plant pathogens to applications during wine fermentation and ageing (inhibition of Botrytis cinerea, Brettanomyces bruxellensis, etc.).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available