Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 6, Pages 1124-1127Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc08726d
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Funding
- National Natural Science Foundation of China [21335005, 21472120, 21622507]
- rogram for Changjiang Scholars and Innovative Research Team in University [IRT_ 15R43]
- Natural Science Basic Research Plan of Shaanxi Province [2015KJXX-22]
- Fundamental Research Funds for the Central Universities [GK201501003, GK201603039]
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Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.
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