Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 87, Issue 4, Pages 315-317Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2017.01.005
Keywords
Class A beta-lactamase; Klebsiella species; Molecular identification; bla(LEN); bla(SHV); bla(OKP)
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Funding
- FAPERJ fellowship
- PNPD-CAPES fellowship
- CNPq
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Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla(SHV), bia(LEN) and bla(OKP) and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine. (C) 2017 Elsevier Inc. All rights reserved.
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