Journal
POLYMERS
Volume 9, Issue 9, Pages -Publisher
MDPI AG
DOI: 10.3390/polym9090399
Keywords
mannoglucan; polysaccharide; Dendrobium nobile Lindl; neuritogenesis; neurodegeneration disease
Categories
Funding
- Project of the Shanghai Committee of Science and Technology [15495800300]
- State Key Program of National Natural Science Foundation of China [31230022]
- Program of Shanghai Subject Chief Scientist [16XD1404500]
- National Natural Science Foundation of China (NSFC) [31670814]
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A water-soluble polysaccharide (JCS1) was isolated from the stems of Dendrobium nobile Lindl. JCS1 was structurally characterized using a combination of chemical and spectral analysis, including methylation analysis, partial acid hydrolysis, Fourier-transform infrared (FTIR) spectroscopy, gas chromatography (GC), GC-mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. The molecular weight was estimated to be 2.3 x 10(4) Da using high-performance gel permeation chromatography (HPGPC). The sugar composition analysis indicated it was composed of glucose, mannose, xylose, and arabinose in a 40.2:2.3:1.7:1.0 molar ratio. The structure analysis showed that JCS1 was a mannoglucan with a backbone consisting of (1 -> 4)-linked beta-Manp and (1 -> 4)-linked alpha-Glcp with branches at C-6 of (1 -> 4)-linked alpha-Glcp residues. The branches were composed of T-alpha-Glcp, 1,4-alpha-Xylp, and T-alpha-Araf. In vitro bioactivity tests revealed that the acetylated derivative of JCS1, YJCS1, induced neuritogenesis of PC-12 cells. These results demonstrate that YJCS1 might be a promising bioactive polysaccharide for development as a drug candidate for the possible prevention and treatment of neurodegeneration diseases.
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