Journal
ACS NANO
Volume 11, Issue 4, Pages 3943-3949Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b00373
Keywords
aptasensor; exosomes; nanotetrahedron; expanded nucleotide; electrochemistry
Categories
Funding
- National Key Scientific Program of China [2011CB911000]
- NSFC [21505032, 21325520, 21327009, 51673012]
- China National Instrumentation Program [2011YQ03012412]
- China Scholarship Council
- Beijing Municipal Science and Technology Commission [D161100002116002]
- National Key Research and Development Program of China [2016YFF0203703]
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Exosomes are extracellular vesicles (50-100 nm) circulating in biofluids as intercellular signal transmitters. Although the potential of cancerous exosomes as tumor biomarkers is promising, sensitive and rapid detection of exosomes remains, challenging. Herein, we combined the strengths of advanced aptamer technology, DNA-based nano structure, and portable electrochemical devices to develop a nanotetrahedron (NTH)-assisted aptasensor for direct capture and detection of hepatocellular exosomes. The oriented immobilization of aptamers significantly improved the accessibility of an artificial nucleobase-containing aptamer to suspended exosomes, and the NTH-assisted aptasensor could detect exosomes with 100-fold higher sensitivity when compared to the single-stranded aptamer-functionalized aptasensor. The present study provides a proof-of-concept for sensitive and efficient quantification of tumor-derived exosomes. We thus expect the NTH-assisted electrochemical aptasensor to become a powerful tool for comprehensive exosome studies.
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