Journal
ACS OMEGA
Volume 2, Issue 2, Pages 443-454Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.6b00495
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Funding
- National Natural Science Foundation of China [31525009, 31271021]
- National High-Tech Project of China (863-Project) [2015AA020316]
- Sichuan Innovative Research Team Program for Young Scientists [2016TD0004]
- International Science & Technology Cooperation Program of China [2013DFG52300]
- Distinguished Young Scholars of Sichuan University [2011SCU04B18]
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A great interest has been shown in the injectable scaffolds for cartilage tissue regeneration because it can fill irregularly shaped defects easily through minimally invasive surgical treatments. Herein, we developed a new injectable three-dimensional (3D) alginate hydrogel loaded with biodegradable porous poly(e-caprolactone)-b-poly( ethylene glycol)-b-poly(e-caprolactone) microspheres (MPs/Alg) as the calcium gluconate container to cross-link alginate. Suspensions of chondrocytes/alginate and porous microspheres turned into a gel because of the release of calcium gluconate; thus, the injectable composite hydrogels give a 3D scaffold to fit the defects perfectly and integrate the extracellular-matrix-mimicking architecture to efficiently accommodate cartilage cells in situ. Tissue repair in a full-thickness cartilage defect model was controlled at 6, 12, and 18 weeks after the implant by micro-CT and immunohistochemistry to evaluate the healing status. The defect in the MPs/Alg+ cells group achieved an almost complete repair at 18 weeks, and the repaired chondrocytes regained a normal tissue structure. Moreover, the MPs/Al+ cells-treated group increased the quality of tissue formed, including the accumulated glycosaminoglycan and the uniformly deposited type II collagen. The results point out the promising application of the injectable MPs/Alg-chondrocytes system for cartilage tissue engineering.
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