Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 13, Pages 11546-11556Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b01850
Keywords
AIE micelles; gemini lipids; nonviral gene vectors; micelle and nanoparticle transition; gene delivery tracing
Funding
- Natural Science Foundation of China [21372032, 91227109]
- Fundamental Research Funds for the Central Universities, Beijing Municipal Commission of Education
- Program for Changjiang Scholars and Innovative Research Team in University
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Four gemini amphiphiles decorated with triazole-[12]aneN(3) as the hydrophilic moiety and various long hydrocarbons as hydrophobic moieties, 1-4, were designed to form micelles possessing the aggregation-induced emission (AIE) property for gene delivery and tracing. All four amphiphiles give ultralow critical micelle concentrations, are pH-/photostable and biocompatible, and completely retard the migration of plasmid DNAs at low concentrations. The DNA-binding abilities of the micelles were fully assessed. The coaggregated nanoparticles of 1-4 with DNAs could convert back into AIE micelles. In vitro transfections indicated that lipids 1 and 2 and their originated liposomes bearing decent delivering abilities have great potentials as nonviral vectors. Finally, on the basis of the transfection and the transitions between condensates and micelles, lipid 2 was singled out as the first example for real-time tracing of the intracellular deliveries of nonlabeled DNA, which provides spatiotemporal messages about the processes of condensate uptake and DNA release.
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