High efficiency succinic acid production from glycerol via in situ fibrous bed bioreactor with an engineered Yarrowia lipolytica

In this study, in situ fibrous bed bioreactor (isFBB) was developed at the first time for efficient succinic acid (SA) production by Yarrowia lipolytica. After optimization, SA titer, productivity and yield of 51.9 g/L, 1.46 g/L/h and 0.42 g/g were obtained respectively via isFBB fermentation under conditions of 750 cm(2) cotton towel, 120 g/L initial glycerol and 3 L/min aeration rate. By fed batch strategy, SA titer raised up to 198.2 g/L was achieved, which was the highest value ever reported. In operation stability study, SA productivity showed no obvious decrease after 12 repeated batches of 460 h fermentation, and cell viability even recovered within two repeated batches after intentional interruption. This study successfully attained a highly efficient and stable isFBB for enhanced SA production by Y. lipolytica. (C) 2016 Elsevier Ltd. All rights reserved.