Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 56, Issue 14, Pages 4052-4055Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201611729
Keywords
DNA nanotechnology; dSTORM; multiplexing; SIM; STED
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Funding
- DFG through an Emmy Noether Fellowship [DFG JU 2957/1-1, SFB 1032]
- ERC through an ERC Starting Grant (MolMap) [680241]
- Max Planck Society
- Max Planck Foundation
- Center for Nanoscience (CeNS)
- Nanoinitiative Munich (NIM)
- International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS-LS)
- DFG through the Graduate School of Quantitative Biosciences Munich (QBM)
- National Institute of Health [1-U01-MH106011-01, 1R01EB018659-01]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1317694] Funding Source: National Science Foundation
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Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20 x higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.
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