Journal
DEVELOPMENT
Volume 144, Issue 6, Pages 998-1007Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.140905
Keywords
Cell culture; Engineer; Model; Niche; Organoid; Stem
Categories
Funding
- Damon Runyon Cancer Research Foundation [DRG-2239-15]
- Congressionally Directed Medical Research Programs [W81XWH-10-1-1-1023, W81XWH-13-1-0221]
- National Institutes of Health [DP2HD080351-01]
- National Science Foundation [DBI-1548297, MCB-1330864]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1330864] Funding Source: National Science Foundation
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For many tissues, single resident stem cells grown in vitro under appropriate three-dimensional conditions can produce outgrowths known as organoids. These tissues recapitulate much of the cell composition and architecture of the in vivo organ from which they derive, including the formation of a stem cell niche. This has facilitated the systematic experimental manipulation and single-cell, high-Jthroughput imaging of stem cells within their respective niches. Furthermore, emerging technologies now make it possible to engineer organoids from purified cellular and extracellular components to directly model and test stem cell-niche interactions. In this Review, we discuss how organoids have been used to identify and characterize stem cell-niche interactions and uncover new niche components, focusing on three adult-derived organoid systems. We also describe new approaches to reconstitute organoids from purified cellular components, and discuss how this technology can help to address fundamental questions about the adult stem cell niche.
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