Journal
DEVELOPMENTAL CELL
Volume 41, Issue 2, Pages 195-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2017.03.024
Keywords
-
Categories
Funding
- CIHR [123513]
- NSERC [312460-2012]
- NIH [R01 GM097576, R24 OD016474]
- MSK Cancer Center Support/Core Grant [P30 CA008748]
Ask authors/readers for more resources
Formation and resolution of multicellular rosettes can drive convergent extension (CE) type cell rear-rangements during tissue morphogenesis. Rosette dynamics are regulated by both planar cell polarity (PCP)-dependent and -independent pathways. Here we show that CE is involved in ventral nerve cord (VNC) assembly in Caenorhabditis elegans. We show that a VANG-1/Van Gogh and PRKL-1/Prickle containing PCP pathway and a Slit-independent SAX-3/Robo pathway cooperate to regulate, via rosette intermediaries, the intercalation of post-mitotic neuronal cell bodies during VNC formation. We show that VANG-1 and SAX-3 are localized to contracting edges and rosette foci and act to specify edge contraction during rosette formation and to mediate timely rosette resolution. Simultaneous loss of both pathways severely curtails CE resulting in a shortened, anteriorly displaced distribution of VNC neurons at hatching. Our results establish rosette-based CE as an evolutionarily conserved mechanism of nerve cord morphogenesis and reveal a role for SAX-3/Robo in this process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available