Journal
BIOSENSORS & BIOELECTRONICS
Volume 92, Issue -, Pages 234-240Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.02.023
Keywords
Biosensor; Biointerface; Influrenza virus; Molecular recognition; Label-free
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Funding
- Impulsing Paradigm Change through Disruptive Technologies
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A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Ac alpha(2-6)Gal in humans, Neu5Ac alpha(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Ac alpha(2-6)Gal beta(1-4)G1c, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.
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