Journal
BIOSENSORS & BIOELECTRONICS
Volume 92, Issue -, Pages 87-94Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.02.002
Keywords
Paper-based microfluidic devices; Photolithography; Electrochemical immunofiltration; Point-of-care testing; Human chorionic gonadotropin
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Funding
- National Key Technologies R & D Program of China [2013BAI03B01]
- National Natural Science Foundation of China [61627807, 61271119]
- Education Department of Guangxi [2013YB090]
- Hunan Provincial Innovation Foundation for Postgraduate [CX2014B043]
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An electrochemical immunofiltration analysis was introduced into microfluidic paper-based analytical devices (mu PADs) for the first time, which was based on photolithography and screen-printing technology. The hydrophilic test zones of the aldehyde-functionalized screen-printed electrodes (SPEs) were biofunctionalized with capture antibodies (Ab(1)). A sensitive immune detection method was developed by using primary signal antibody functionalized gold nanoparticles (GNPs/Ab(2)) and alkaline phosphatase conjugated secondary antibody (ALP-IgG). Differential pulse voltammetry (DPV) was performed to detect the electrochemical response. The microfluidic paper-based electrochemical immunosensor (mu-PEI) was optimized and characterized for the detection of human chorionic gonadotropin (HCG), a model analyte, in a linear range from 1.0 mIU mL(-1) to 100.0 IU mL(-1) with a detection limit of 0.36 mIU mL(-1). Additionally, the proposed mu-PEI was used to test HCG in real human serum and obtained satisfactory results. The disposable, efficient, sensitive and low-cost mu-PEI has exhibited great potential for the development of point-of-care testing (POCT) devices that can be applicated in healthcare monitoring.
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