Journal
CLINICAL AND EXPERIMENTAL ALLERGY
Volume 47, Issue 4, Pages 488-498Publisher
WILEY
DOI: 10.1111/cea.12876
Keywords
allergen challenge; CD32; degranulation; eosinophils; IL-3
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Funding
- National Institutes of Health [P01 HL088594, UL1 RR025011]
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Background Eosinophils contribute to the pathogenesis of multiple diseases, including asthma. Treatment with antibodies targeting IL-5 or IL-5 receptor a reduces the frequency of asthma exacerbations. Eosinophil receptors for IL-5 share a common a-chain with IL-3 and GM-CSF receptors. We recently reported that IL-3 is more potent than IL-5 or GMCSF in maintaining the ERK/p90S6K/RPS6 ribosome-directed signaling pathway, leading to increased protein translation. Objective We aimed to determine disease-relevant consequences of prolonged eosinophil stimulation with IL-3. Results Human blood eosinophils were used to establish the impact of activation with IL-3 on IgG-driven eosinophil degranulation. When compared to IL-5, continuing exposure to IL-3 further induced degranulation of eosinophils on aggregated IgG via increased production and activation of both CD32 (low affinity IgG receptor) and alpha M beta 2 integrin. In addition, unlike IL-5 or GM-CSF, IL-3 induced expression of CD32B/C (FCGRIIB/C) subtype proteins, without changing CD32A (FCGRIIA) protein and CD32B/C mRNA expression levels. Importantly, these in vitro IL-3-induced modifications were recapitulated in vivo on airway eosinophils. Conclusions and Clinical Relevance We observed for the first time upregulation of CD32B/C on eosinophils, and identified IL-3 as a potent inducer of CD32-and alpha M beta 2-mediated eosinophil degranulation.
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