Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 40, Issue 4, Pages 510-515Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b16-00945
Keywords
speciation; zinc (Zn) complex; dual label; biodistribution; gamma-ray emission imaging (GREI)
Categories
Funding
- [26.8219]
Ask authors/readers for more resources
Many zinc (Zn) complexes have been developed as promising oral antidiabetic agents. In vitro assays using adipocytes have demonstrated that the coordination structures of Zn complexes affect the uptake of Zn into cells and have insulinomimetic activities, for which moderate stability of Zn complexes is vital. The complexation of Zn plays a major role improving its bioavailability. However, investigation of the speciation changes of Zn complexes after oral administration is lacking. A dual radiolabeling approach was applied win order to investigate the speciation of bis(5-chloro-7-iodo-8-quinolinolato)zinc complex [Zn(Cq)(2)], which wexhibits the antidiabetic activity in diabetic mice. In the present study, Zn-65- and I-131-labeled [Zn(Cq)(2)] were synthesized, and their biodistribution were analyzed after an oral administration using both invasive conventional assays and noninvasive gamma-ray emission imaging (GREI), a novel nuclear medicine imaging modality that enables analysis of multiple radionuclides simultaneously. The GREI experiments visualized the behavior of Zn-65 and [I-131]Cq from the stomach to large intestine and through the small intestine; most of the administered Zn was transported together with clioquinol (5-chloro-7-iodo-8-quinolinol) (Cq). Higher accumulation of Zn-65 for [Zn(Cq)(2)] than ZnCl2 suggests that the Zn associated with Cq was highly absorbed by the intestinal tract. In particular, the molar ratio of administered iodine to Zn decreased during the distribution processes, indicating the dissociation of most [Zn(Cq)(2)] complexes. In conclusion, the present study successfully evaluated the speciation changes of orally administered [Zn(Cq)(2)] using the dual radiolabeling method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available