Journal
HUMAN GENE THERAPY METHODS
Volume 28, Issue 5, Pages 235-246Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/hgtb.2017.192
Keywords
adeno-associated virus (AAV); viral vector; vector purification; titration; quality control
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Funding
- Bundesministerium fur Bildung und Forschung [01GU0527]
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Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure that results in well-characterized vectors suitable for both in vitro approaches and preclinical studies.
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