Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 77, Issue 5, Pages -Publisher
WILEY
DOI: 10.1111/aji.12648
Keywords
acute chorioamnionitis; apoptosis-associated speck-like protein containing a CARD (ASC); ASCspeck; caspase-1; interleukin-1 beta; intra-amniotic infection/inflammation
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Funding
- Eunice Kennedy Shriver National Institute of Child Health and Human Development [HHSN275201300006C]
- National Institutes of Health
- Department of Health and Human Services
- Wayne State University Perinatal Initiative in Maternal, Perinatal and Child Health
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Problem: Inflammasome activation requires two steps: priming and assembly of the multimeric complex. The second step includes assembly of the sensor molecule and adaptor protein ASC (an apoptosis-associated speck-like protein containing a CARD), which results in ASC speck formation and the recruitment of caspase (CASP)-1. Herein, we investigated whether there is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes from women who underwent spontaneous labor at term. Method of Study: Using in situ proximity ligation assays, ASC/CASP-1 complexes were determined in the chorioamniotic membranes from women who delivered at term without labor or underwent spontaneous labor at term with or without acute histologic chorioamnionitis (n=10-11 each). Also, ASC speck formation was determined by flow cytometry in the choriodecidual leukocytes isolated from women who delivered at term with or without spontaneous labor (n=9-12 each). Results: (i) ASC/CASP-1 complexes were detected in the chorioamniotic membranes; (ii) ASC/CASP-1 complexes were greater in the chorioamniotic membranes from women who underwent spontaneous labor at term than in those without labor; (iii) ASC/CASP-1 complexes were even more abundant in the chorioamniotic membranes from women who underwent spontaneous labor at term with acute histologic chorioamnionitis than in those without this placental lesion; (iv) ASC speck formation was detected in the choriodecidual leukocytes; and (v) ASC speck formation was greater in the choriodecidual leukocytes isolated from women who underwent spontaneous labor at term than in those without labor. Conclusion: There is inflammasome assembly in the chorioamniotic membranes and choriodecidual leukocytes during spontaneous labor at term.
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