Journal
CANCER RESEARCH
Volume 77, Issue 9, Pages 2339-2350Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-16-1883
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Funding
- Ministry of Science and Technology of Taiwan [MOST 104-2320-B-006-036-MY3]
- National Health Research Institute [NHRI-EX102-10244BI]
- Top University Grant of National Cheng Kung University [D103-35A17]
- Cancer Prevention Research Institute of Texas [RP160283]
- Duncan Cancer Center
- Cancer Prevention Research Institute of Texas HIHRRA [RP160795]
- National Institutes of Health [R01EB013584]
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Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-of-function studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis. (C) 2017 AACR.
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