Journal
BIOLOGICAL CHEMISTRY
Volume 398, Issue 5-6, Pages 687-699Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2017-0104
Keywords
AQUA; label-free; LC-MS/MS; prenylation; protein-protein interactions; SILAC
Categories
Funding
- German Research Foundation [SFB642]
Ask authors/readers for more resources
Quantitative mass spectrometry approaches are used for absolute and relative quantification in global proteome studies. To date, relative and absolute quantification techniques are available that differ in quantification accuracy, proteome coverage, complexity and robustness. This review focuses on most common relative or absolute quantification strategies exemplified by three experimental studies. A label-free relative quantification approach was performed for the investigation of the membrane proteome of sensory cilia to the depth of olfactory receptors in Mus musculus. A SILAC-based relative quantification approach was successfully applied for the identification of core components and transient interactors of the peroxisomal importomer in Saccharomyces cerevisiae. Furthermore, AQUA using stable isotopes was exemplified to unraveling the prenylome influenced by novel prenyltransferase inhibitors. Characteristic enrichment and fragmentation strategies for a robust quantification of the prenylome are also summarized.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available