Journal
BIORESOURCE TECHNOLOGY
Volume 231, Issue -, Pages 75-84Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.01.051
Keywords
Succinate; Glycerol; Acetate; Escherichia coli; Two-stage fermentation
Funding
- National High Technology Research and Development Program of China [2011AA02A203]
- National Science Foundation for Young Scientist of China [21406065]
- Fundamental Research Funds for the Central Universities [222201313007, 22A201514042]
- Open Funding Project of the State Key Laboratory of Bioreactor Engineering
- Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education [2015101]
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Glycerol is an important resource for production of value-added bioproducts due to its large availability from the biodiesel industry as a by-product. In this study, two metabolic regulation strategies were applied in the aerobic stage of a two-stage fermentation to achieve high metabolic capacities of the pflB ldhA double mutant Escherichia coli strain overexpressing phosphoenolpyruvate carboxykinase (PCK) in the subsequent anaerobic stage: use of acetate as a co-carbon source of glycerol and restriction of oxygen supply in the PCK induction period. The succinate concentration achieved 926.7 mM with a yield of 0.91 mol/mol during the anaerobic stage of fermentation in a 1.5-L reactor. qRT-PCR indicated that the two strategies enhanced transcription of genes related with glycerol metabolism and succinate production. Our results showed this metabolically engineered E.coli strain has a great potential in producing succinate using glycerol as carbon source. (C) 2017 Elsevier Ltd. All rights reserved.
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