Journal
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Volume 27, Issue -, Pages 37-43Publisher
UNIV CATOLICA DE VALPARAISO
DOI: 10.1016/j.ejbt.2017.03.001
Keywords
Biochemical properties; Food biotechnology; Fungal beta-galactosidases; Glycosyl hydrolase family; Recombinant Pichia pastoris; Transgalactosylation reactions; beta-Galactosidase characterization; beta-Galactosidase cloning; beta-Galactosidase expression; beta-Galactosidase family
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Funding
- National Natural Science Foundation of China [31601407, 31370076]
- National Natural Science Foundation of Fujian Province, China [2016J01157]
- Priming Scientific Research Foundation of Fuzhou University [XRC-1564]
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Background: beta-Galactosidases catalyze both hydrolytic and transgalactosylation reactions and therefore have many applications in food, medical, and biotechnological fields. Aspergillus niger has been a main source of beta-galactosidase, but the properties of this enzyme are incompletely studied. Results: Three new beta-galactosidases belonging to glycosyl hydrolase family 35 from A. niger F0215 were cloned, expressed, and biochemically characterized. In addition to the known activity of LacA encoded by lacA, three putative beta-galactosidases, designated as LacB, LacC, and LacE encoded by the genes lacB, lacC, and lacE, respectively, were successfully cloned, sequenced, and expressed and secreted by Pichia pastoris. These three proteins and LacA have N-terminal signal sequences and are therefore predicted to be extracellular enzymes. They have the typical structure of fungal beta-galactosidases with defined hydrolytic and transgalactosylation activities on lactose. However, their activity properties differed. In particular, LacB and lacE displayed maximum hydrolytic activity at pH 4-5 and 50 degrees C, while LacC exhibited maximum activity at pH 3.5 and 60 degrees C. All beta-galactosidases performed transgalactosylation activity optimally in an acidic environment. Conclusions: Three new beta-galactosidases belonging to glycosyl hydrolase family 35 from A. niger F0215 were cloned and biochemically characterized. In addition to the known LacA, A. niger has at least three beta-galactosidase family members with remarkably different biochemical properties. (C) 2017 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
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