Journal
EMBO JOURNAL
Volume 36, Issue 9, Pages 1261-1278Publisher
WILEY
DOI: 10.15252/embj.201694561
Keywords
Arabidopsis; BRCA1; DNA damage response; E2FA; RETINOBLASTOMA RELATED
Categories
Funding
- Marie-Curie IEF fellowship [FP7-PEOPLE-2012-IEF 330789, FP7-PEOPLE-2012-IEF 330713]
- Netherlands Organization for Scientific Research, Spinoza grant
- Utrecht University Focus Mass grant
- CBSG2/NCSB
- BBSRC-NSF [BB/M025047/1]
- Grant Agency of the Czech Republic [15-11657S P501]
- Hungarian Academy
- OTKA [107838]
- Campus Hungary [TAMOP-4.2.4B/2-11/1-2012-0001]
- Ministry for National Economy (Hungary) [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-15-2016-00032, GINOP-2.3.2-15-2016-00020]
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
- ERA-CAPS grant [2013/15548/ALW]
- BBSRC [1813952, BB/M025047/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M025047/1, 1813952] Funding Source: researchfish
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The rapidly proliferating cells in plant meristems must be protected from genome damage. Here, we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED (RBR) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2FA are recruited to heterochromatic gamma H2AX-labelled DNA damage foci in an ATM- and ATR-dependent manner. These gamma H2AX-labelled DNA lesions are more dispersedly occupied by the conserved repair protein, AtBRCA1, which can also co-localise with RBR foci. RBR and AtBRCA1 physically interact in vitro and in planta. Genetic interaction between the RBR-silenced amiRBR and Atbrca1 mutants suggests that RBR and AtBRCA1 may function together in maintaining genome integrity. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.
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