Journal
CHEMPLUSCHEM
Volume 82, Issue 3, Pages 337-341Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.201600651
Keywords
DNA; gated materials; Mycoplasma detection; nanoporous anodic alumina
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Funding
- Spanish Government [MAT2015-64139-C41-R, AGL2015-70235-C2-2-R, TEC2015-71324-R]
- Generalitat Valenciana [PROMETEOII/2014/047]
- Catalan authority [AGAUR 2014SGR1344]
- ICREA under the 2014 ICREA Academia Award for support
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A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of molecular gates selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies mu L-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.
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