Journal
CELL REPORTS
Volume 20, Issue 10, Pages 2513-2524Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2017.08.047
Keywords
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Categories
Funding
- Wellcome Trust [095668, 095669]
- Medical Research Council Doctoral Training Award
- Human Frontiers Science Program Fellowship [LT001071]
- Sir Henry Wellcome Fellowship [106101]
- Marie Sklodowska-Curie fellowships [656528, 623872]
- Simons Foundation [SCGB 325476]
- Champalimaud Foundation
- Fundacao para Ciencia e Tecnologia [SFRH/BD/51895/2012]
- Wellcome Trust
- Wellcome Trust [106101/Z/14/Z] Funding Source: Wellcome Trust
- BBSRC [BB/P007201/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/P007201/1, BB/P003273/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1801807] Funding Source: researchfish
- Wellcome Trust [106101/Z/14/Z] Funding Source: researchfish
- Marie Curie Actions (MSCA) [656528] Funding Source: Marie Curie Actions (MSCA)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/51895/2012] Funding Source: FCT
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Research in neuroscience increasingly relies on the mouse, a mammalian species that affords unparalleled genetic tractability and brain atlases. Here, we introduce high-yield methods for probing mouse visual decisions. Mice are head-fixed, facilitating repeatable visual stimulation, eye tracking, and brain access. They turn a steering wheel to make two alternative choices, forced or unforced. Learning is rapid thanks to intuitive coupling of stimuli to wheel position. The mouse decisions deliver high-quality psychometric curves for detection and discrimination and conform to the predictions of a simple probabilistic observer model. The task is readily paired with two-photon imaging of cortical activity. Optogenetic inactivation reveals that the task requires mice to use their visual cortex. Mice aremotivated to performthe task by fluid reward or optogenetic stimulation of dopamine neurons. This stimulation elicits a larger number of trials and faster learning. These methods provide a platform to accurately probe mouse vision and its neural basis.
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